Evaluating the Degradation Process of Collagen Sponge and Acellular Matrix Implants In Vivo Using the Standardized HPLC-MS/MS Method

The purpose of this study was to establish a collagen determination method based on an isotope-labeled peptide as internal reference via high-performance liquid chromatography–tandem mass spectrometry (HPLC–MS/MS), and using the established evaluate degradation process collagen-based implants in vivo. specific (GPAGPQGPR) bovine type I identified with Orbitrap spectrometer. Then, quantification detection HPLC-MS/MS validated, then further used analyze trend sponge acellular matrix (ACM) vivo at 2, 4, 6, 8, 12, 16, 18 weeks after implantation. results indicate that relative standard deviation (RSD) precision repeatability peptide-based were 3.55% 0.63%, respectively. limitations 2.05 × 10−3 μg/mL 1.12 μg/mL, ACM completely degraded 10 weeks, Conclusion: A spectrometer, standardized HPLC-MS/MS-based for established. can be analysis